Single cell trajectory mapping of human pluripotent stem cells differentiating towards lung and hepatocyte progenitors. Single cell trajectory mapping of human pluripotent stem cells differentiating towards lung and hepatocyte progenitors

The molecular details for the specification of lung progenitors from human pluripotent stem cells (hPSCs) are unclear. Here, we use single cell RNA-sequencing with high temporal resolution along an optimized differentiation protocol to determine the transcriptional hierarchy of lung specification from human hPSCs. To chart a comprehensive map of the transcriptional states underlying the differentiation of lung progenitors and hepatoblasts in parallel, we performed a 16-day time-series scRNA-seq using the Drop-Seq workflow (Macosko et al., 2015). Single cell suspensions were processed daily, resulting in the analysis of a total of 10,667 cells that were used for downstream analysis. Overall design: Single-cell transcriptomics on human induced stem cell during early differentiation