Transcriptome high-throughput sequencing of ESRP1-overexpressing cells ESRP1-SKOV3

ESRP1 is an epithelial-specific splicing factor. It mainly regulates expressions of genes related to intercellular adhesion, actin cytoskeleton, cell polarity and cell migration at the post-transcriptional level by alternative splicing. It also plays an important role in the development and progression of cancers. This study analyzed the transcriptome changes of ESRP1 stably overexpression SKOV3 cells by high-throughput sequencing, discovered and validated the functional effects of ESRP1 on ovarian cancer cells Overall design: We constructed ESRP1 stably overexpression cell line ESRP1-SKOV3 and control group EV-SKOV3 using lentivirus.Total RNA was extracted from ESRP1-SKOV3 and EV-SKOV3 after routine cell culture, three copies per group. After high-throughput sequencing of RNA from both groups, differentially expressed genes and alternative splicing differential genes were obtained.