Genome wide analysis of the effect of MMP13-ablation on mouse dental pulp tissue

Tissue form 3-month old mouse molar teeth was used to obtain mRNA profies in developing wild-type (WT) and matrix metalloproteinase-13 (Mmp13−/−) mice using high throuput RNA-seq.Ssamples were analysed in triplicate using Illumina HiSeq 2500 system. Sequence reads that passed stringent quality filters were analyzed at the transcript isoform level using after passing a t-test (P < 0.05), post-hoc test (Storey with Bootstrapping) with a corrected q value of 0.05. qRT–PCR validation was performed using SYBR Green assays. the effect of MMp13 on tooth development and mineralsiation processes was the focus of this work. Dental pulp mRNA profiles of 3-month old wild type (WT) and Mmp13-/- mice. 3 Biological replicates per genotype.