Figure 2. Bifidobacterium alters DC and Macrophage cytokine secretion.
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<strong>Figure 2</strong>. <em><strong>Bifidobacterium </strong></em><strong>alters DC and MF cytokine secretion.</strong> DCs <strong>(A-C)</strong> or MF <strong>(D-F)</strong> stimulated with EPS or UV-killed ATCC25526 (ATCC) or UMB-MBP-01 (MD), and 24 hrs later supernatants analyzed for <strong>A, D)</strong> IL-6, <strong>B, E)</strong> TNFa, and <strong>C, F)</strong> IL-10 by ELISA. Treatments compared using one-way ANOVA. * p value < 0.05; ** p value < 0.01, *** p value < 0.001, **** p value < 0.0001. UV-killed bacteria data representative of 3 separate experiments, 2-3 wells/culture condition, 2 technical replicates/well (supernatants from each well split and analyzed in duplicate), i.e. 4-6 wells per condition per experiment, 14 total wells per condition over 3 experiments. EPS data representative of 2 separate experiments, 2-3 wells/culture condition, 2 technical replicates/well (supernatants from each well split and analyzed in duplicate), i.e. 4 wells per condition per experiment, 10 total wells per condition over 2 experiments.
