Myosin-dependent short actin filaments contribute to peripheral widening in developing stereocilia

Airyscan microscopy images:
Fig. 1b_Live imaging of EGFP-actin
Fig. 2a-b_RPEL1-EGFP
Fig. 2c-e_AC15 or JLA20 labeling
Fig. 3a-c_EGFP-TMOD1
Fig. 6_mutant actin_AP or DVD
Fig. 7b_PE in myo3ab mutant
Fig. 7d-e_PE in myo15a mutant
Suppl. 1_early postnatal day OE and prolonged OE
Suppl. 4b-d_PE or BE label in EGFP-actin IHC
Suppl. 5a-b_vanadate treatment
Suppl. 6e_MYO3A staining in myo15a null

Expansion microscopy images:
Fig. 1c_EGFP-actin incorporation pattern
Fig. 7a_Localization of His-TMOD1_MYO3A_MYO15A
Suppl. 7b_Stereocilia width in EGFP-MYO3A OE IHCs

Lattice SIM images:
Fig. 1d_EGFP-actin for FSCN2 pattern
Fig. 3f-h_BE or PE localization
Suppl. 5c-d_MYO3A or MYO15A localization

Leica SP8 images:
Fig. 3d_PE or BE probing
Fig. 4a-b_His-TMOD1 level_LatA
Fig. 4c-f_Postnatal His-TMOD1 labeling_P3-P9
Fig. 5_Stereocilia widen_PE_BE
Fig. 7c_PE in MYO3 mutants
Fig. 7f-g_PE level_OE of MYO3A
Fig. 7h-i_PE level_OE of MYO15A
Suppl. 2_EGFP-SH3BGRL2
Suppl. 3_PE or BE probing_high salt
Suppl. 4a_Actin-IRES-EGFP
Suppl. 6c_MYO15A and PE in myo15a mosaic IHCs
Suppl. 6f_MYO15A stain in myo3 mutant
Suppl. 7a_OE of EGFP-MYO3A-WT

Nikon Ti2-E CSU-X1 images:
Suppl. 6d_His-TMOD1 probing in shaker IHCs