Full-length Transcriptome Analysis of Artemisia tangutica Endemic to Qinghai-Tibetan Plateau

The fresh and tender leaves of Artemisia tangutica for sequencing were collected from Wulan Xian, Mongolian and Tibetan Autonomous Prefecture of Haixi, Qinghai Province (geographic coordinates: N 36 ° 56'37 ", E 98 ° 27'20"; altitude: 2939 meters). After the leaves were collected, they were quickly stored in liquid nitrogen. The voucher specimen (chen2019104) was deposited in the Qinghai Tibet Plateau Biological Herbarium (HNWP) of the Northwest Plateau Institute of Biology, Chinese Academy of Sciences. The total RNA of Artemisia tangutica leaves was extracted by Trizol method. Agarose gel electrophoresis was used to check whether the extracted RNA was contaminated, and the RNA concentration was detected by nanodrop. The quality and integrity of RNA were evaluated by nanophotometer spectrophotometer and Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA). For qualified RNA samples, Oligo DT was used as primer to reverse the target mRNA, and full-length cDNA was amplified by low cycle PCR. The NEBNext End repair/dA tailing Module is used for terminal repair and A addition, and the ONT SQKLSK109 kit and NEBNext Quick Ligation Module are used for connection of sequencing connector. The sequencing platform is PromethION (Oxford Nanopole Technologies, UK).