A comparison of workflows and consumable costs for various viral sequencing approaches.
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The ‘Custom’ protocol refers to the modified RNA-Seq method used in this study to create larger insert fragments. Alternative methods include Amplicon-seq and hybridisation capture (SureSelect Target Enrichment for Illumina Paired-End mRNA-Seq Library Prep; version 1.1). Failure rates are determined by failure to sequence at least one amplicon (*failure rate based on that observed with Sanger Sequencing.**estimated cost for probes only. Extra cost incurred for Agilent library preparation kit plus additional reagents recommended by Agilent.***linear amplification during SPIA reverse transcription has not been accounted for.
创建时间：
2015-12-02
