Tobacco arsenic transport gene NtNIP7-1 and cloning method and applications thereof
收藏国家林业和草原科学数据中心2023-02-12 更新2024-03-07 收录
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A tobacco arsenic transport gene NtNIP7-1 and a cloning method and application thereof are disclosed. A nucleotide sequence of the tobacco arsenic transport gene NtNIP7-1 is shown as SEQ ID: No. 1， and an encoded amino acid sequence thereof is shown as SEQ ID: No. 2. The cloning method of the tobacco arsenic transport gene NtNIP7-1 includes (S1) extracting RNA in tobacco， performing reverse transcription， and obtaining a first-strand cDNA; and (S2) taking the first-strand cDNA obtained by the reverse transcription as a template， synthesizing a specific primer according to sequences of the NtNIP7-1 gene， performing PCR amplification， recovering and purifying a product of the PCR amplification， and sequencing. In the present invention， inhibition of the expression of the tobacco endogenous gene NtNIP7-1 in tobacco plants is able to significantly reduce the arsenic content of tobacco leaves， and has broad application prospects in the field of low arsenic content breeding.
提供机构：
国家林业和草原科学数据中心
创建时间：
2023-02-12
