scRNA-seq for stromal Lkb1 deficiency-induced gastrointestinal polyps

Numerous studies have showed that mesenchymal-specific Lkb1 is sufficient for the development of PJS-like polyps in mice. However, the cellular origin and component of these Lkb1-associated polyps and underlying mechanisms remain elusive. In this present study, we generated tamoxifen-inducible Lkb1flox/flox;Myh11-Cre/ERT2 mice and performed scRNA-seq to investigate the cellular complexity of gastrointestinal polyps associated with PJS. Polyps tissues from three Lkb1flox/+;Myh11-Cre/ERT2 mice and normal duodenum tissues of three Lkb1+/+;Myh11-Cre/ERT2 (WT) mice were collected.