Relative expression of genes associated with NF-κB. RNA was extracted from C3842 cells treated with IL-1β (10 ng/ml), Curcumin (20 µmol/l) or both.

qRT-PCR was performed using the NF-κB primer library (RealTimePrimers, Elkins Park, PA, USA).See Material and Methods section for details. The gene expression levels were normalized to the expression of ribosomal protein 13a (RPL13a). The Curcumin effect on IL-1β-induced gene expression was calculated as ratio of the gene expression in cells treated with IL-1β+Curcumin and cells treated with IL-1β alone. Relative changes higher than 2-fold are marked bold. Relative changes lower than 0.5-fold are marked italic. Putative housekeeping genes other than RPL13a are indicated by italics.