CD73+ epithelial progenitor cells which contribute to homeostasis and renewal are depleted in eosinophilic esophagitis

We performed single-cell RNA sequencing on esophageal PDOs from 4 non-EoE control patients to investigate the molecular heterogeneity of human esophageal organoids. Seurat's unsupervised dimensionality reduction and clustering along with uniform manifold approximation and projection visualization showed 4 distinct cell clusters in esophageal PDOs cultures with or without IL13 Overall design: Patient-derived esophageal organoids from 4 control patients were cultured with or without IL-13 treatment, followed whole cell single cell RNA-seq