Transcriptional response to seawater acidification and hyperthermal stress in the azooxanthellate octocoral, Scleronephthya gracillimum

The soft coral Scleronephthya gracillimum is an azooxanthellate octocoral order Alcyonacea. In this study, stress responses to increased seawater temperature and marine acidification were investigated using a microarray. The S. gracillimum microarray was constructed. The S. gracillimum microarray was constructed after RNA-seq. Oligonucleotides were picked from UniGene of S. gracillimum and the clones were annotated using Blast. For hyperthermal stress experiment consists of 24C vs. 26C, 24C vs. 28C. Each group has 3 individual corals. The experiment were duplicate. For acidification experiment consists of pH6.5, pH7.0, pH 7.5 groups and each group has 3 individual corals. For hyperthermal and acidification dual stress experiments consist of 8.0 vs. 7.0, 8.0 vs. 7.5 and 8.0 vs. 7.8 groups and each group has 3 individual corals and the experiments were duplicated.