Transcriptional profiling in adult onset ADPKD mouse model based on Pkd2 inactivation

We performed unbiased transcriptional profiling in an adult-onset Pkd2 mouse model at an early stage before cysts formed using as controls wild type and PC2+cilia double knockout mice which are protected from ADPKD. By a tripartite comparison, we identified differetially expressed genes which have significant different expression in Pkd2-only knockout with same direction of change from both wild type and Pkd2+Cilia double knockout. Overall design: RNA-Seq profiling using kidneys from Pkd2 knockout and controls wild type and Pkd2+cilia double knockout mice at 10 weeks old with three biological replicates for each genotype were performed.