RNA-seq analysis of proximal tubule specific Fan1 knockout transgenic kidneys subjected to cisplatin injury

FAN1 is a DNA endonuclease that we have previously identified as the underlying genetic cause of karyomegalic interstitial nephritis (OMIM: 614817) in humans. In order to deduce the molecular function of FAN1 in the setting of acute kidney injury and progression to chronic kidney disease, we generated a proximal tubule specific Fan1 knockout mouse model and subjected these mice to cisplatin injury. RNA-seq of the transgenic mice kidneys revealed dysregulation of the DNA damage repair pathway as well as cell cycle associated genes. Together, the data supports for a role of Fan1 in DNA damage repair, and that in its absence results in dysfunctional proximal tubule repair and regeneration upon cisplatin injury. Overall design: 2X150 bp standard RNA-seq was performed using an Illumina HiSeq platform