Physiologic conditions at the beginning and end of ventilation.

Arterial blood gases and mean arterial pressure were obtained from non-ventilated mice and mice ventilated at a tidal volume of 6 ml/kg or 30 ml/kg for 4 h (n = 10 per group). No statistical difference was found in pH, PaO2, PaCO2, mean arterial pressure, and peak inspiratory pressure at the beginning versus the end of 4 h of mechanical ventilation. The normovolemic statuses of mice were maintained by monitoring mean artery pressure. Because no differences were observed between control, non-ventilated mice with or without iPSCs, with or without nontargeting scrambled siRNA, with or without SN50, with or without NKRF siRNA, with or without heat-inactivated conditioned medium, mice ventilated at 30 ml/kg with or without mouse embryonic fibroblasts, mice ventilated at 30 ml/kg with iPSCs or conditioned medium, the data were combined. CM = conditioned medium of iPSCs; FiO2 = fraction of inspired oxygen; iPSCs = induced pluripotent stem cells; MAP = mean arterial pressure; NKRF siRNA = nuclear factor-κB repressing factor short interfering RNA; PIP = peak inspiratory pressure; SN50 = NF-κB inhibitor; Scam = nontargeting scrambled siRNA; VT = tidal volume. The physiological data of control groups were similar during the experiment and were used as the beginning data of ventilation.