Physiological transcriptional profiling of murine osteocytes from calvaria and long bones

Different bones of the skeleton originate from three distinct embryonic lineages. Osteoblasts derived from bones of different embryonic origin displayed cell intrinsic difference. Osteocytes are terminally differentiated osteoblasts which displays a unique genetic makeup and a distinct morphology. It is not know if osteocytes display a cell intrinsic differences depeding on their origin. To understand the difference in the gene expression profiles of osteocytes of three different embryonic origins we examined osteocytes from three different bones neural crest origin-frontal bone, paraxial mesoderm origin-parietal bone, and lateral plate mesoderm- femur bone. Examine the changes in the transcriptome in frontal, parietal and femur osteocytes.