Fast single-cell joint analysis of histone modifications and transcriptome by Droplet Paired-Tag

Analysis of histone modifications at single-cell resolution can provide insights into the cellular heterogeneity in activity states of regulatory elements. However, current methods are hindered by lengthy procedures and insufficient sensitivity. Here we present Droplet Paired-Tag, a microfluidic cell barcoding-based method for fast and robust joint analysis of histone modifications and gene expression from single cells. We applied Droplet Paired-Tag to mouse brain and demonstrated its utility in accurately identifying active or repressive regulatory elements, and resolving the dynamic interactions between candidate regulatory elements and putative target genes. Combined analysis of RNA and histone modifcations in single-cells单细胞分辨率下的组蛋白修饰分析，可揭示调控元件活性状态中的细胞异质性。然而现有分析方法受限于流程冗长、灵敏度不足的缺陷。本研究提出液滴配对标签（Droplet Paired-Tag）技术，这是一种基于微流控细胞条码标记的方法，可实现单细胞水平组蛋白修饰与基因表达的快速、稳健联合分析。我们将液滴配对标签技术应用于小鼠脑组织，证实其可精准识别活性或抑制型调控元件，并解析候选调控元件与潜在靶基因之间的动态互作关系。单细胞水平RNA与组蛋白修饰的联合分析