NK cell response to IL2 in vitro

Here we analyzed the transcriptional response to IL2 in NK cells in vitro. Splenic NK cells were isolated by negative selection from 6wk old male C57BL/6 mice. These were directly cultured with IL2 (250U/ml) for 8hrs in vitro. Cells were then transferred directly into TriZol. RNA was prepared in Trizol for gene expression profiling by Affymetrix Mouse Gene 1.0 ST Arrays. The samples in this series are re-analyzed samples from GSE84562.