Summary of spots detected by Same Spots Software.

Total number: Number of spots detected by Same Spots Software after filtering (area size and minimum volume).Differentially expressed: Number of spots where difference of expression (normalized volume) of two samples in the experimental set meet the statistical criteria of ANOVA≤0.05.Excluded: Number of spots that were excluded.Included: Number of “Differentially expressed” minus “Excluded”.Picked: Number of spots picked and trypsinized for mass spec analysis.w/o ID: Number of spots where no peptide could be identified.Proteins Identified: Total number of Proteins identified in all spots picked in experimental set. Note that more than one protein can be identified in a single picked spot.