Essentiality of eccC5 and analysis of functional domains.

* The eccC5 NBD mutants appear to have a dominant negative effect on the functioning of endogenous EccC5.$ Colonies showed a strong growth defect, i.e. colonies were visible only after 17 days, compared to 10 days for the wild-type strain.Replacement of pMV-eccBC5 by the input DNA was scored. Input DNA consisted of the pMV-361-hyg plasmid containing the indicated constructs. “+” indicates that more than 100 colonies were detected after electroporation with the indicated vector. “–” indicates between 0–20 colonies were found after electroporation. These latter colonies were shown by PCR to still contain the original vector, indicating illegitimate recombination or spontaneous antibiotic resistance. Results are representative data of three independent experiments.Essentiality of eccC5 and analysis of functional domains.