Patch-seq of human patient-derived diffuse midline glioma xenografts

Treatment-resistance of lethal high-grade brain tumors including H3K27M diffuse midline gliomas is thought to arise in part from transcriptional and electrophysiological heterogeneity. These phenotypes are readily studied in isolation using single-cell RNA-seq and whole-cell patch clamping, respectively, but their simultaneous capture is now possible by aspirating a cell's contents into a patch pipet after electrophysiology recordings using a method called 'patch-seq'. Here, we adapt this method to characterize the gene expression programs and functional responses of patient-derived glioma xenografts to neuronal firing at single-cell resolution. Xenografted patient-derived diffuse midline glioma cells were analyzed by whole-cell patch clamp with neuronal stimulation and subsequently by single cell RNA-seq ***Raw data not available due to patient privacy concerns***