Differential expression of long noncoding RNAs of dental pulp stem cells in the SDF-1α induced

We triggered the involvement of lncRNAs in odontogenic differentiation of DPSCs by incubation with SDF-1α. By LncRNA microarray, alterations in lncRNA expression at odontogenic differentiation inducted by 100ng/Ml SDF-1α were identified. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to validate several random upregulated and downregulated LncRNAs in the odontogenic differentiation of DPSCs. In addition, Gene ontology (GO) analysis and coding-noncoding gene coexpression (CNC) analysis were conducted to predict the interactions of coding and noncoding RNA and identify core regulatory factors in odontogenic differentiation of DPSCs. SDF-1α induced lncRNAs expression in human dental pulp stem cells was measured at 7 days after exposure to doses of 100ng/mL. Three independent experiments were performed at each group (with or without SDF-1α) for each experiment.