Efficacy and mechanism of action of cipargamin as an antibabesial drug candidate

Babesiosis is a disease brought on by intraerythrocytic parasites of the genus Babesia. Current chemotherapies are accompanied by side effects and parasite relapse. Therefore, it is crucial to develop highly effective drugs against Babesia. Cipargamin (CIP) has shown inhibition against apicomplexan parasites, mainly Plasmodium and Toxoplasma. This study evaluated the growth-inhibiting properties of CIP against Babesia spp. and investigated the mechanism of CIP on B. gibsoni. The half inhibitory concentration (IC50) values of CIP against the in vitro growth of B. bovis and B. gibsoni were 20.2 ± 1.4 nM and 69.4 ± 2.2 nM, respectively. CIP significantly inhibited the growth of B. microti and B. rodhaini in vivo. Resistance was conferred by L921V and L921I mutations in BgATP4, which reduced the sensitivity to CIP by 6.1- and 12.8-fold. The inhibitory potency of CIP against BgATP4-associated ATPase activity was moderately reduced in mutant strains, with a 1.3-fold and 2.4-fold decrease in B..., , **Project Title:** Efficacy and mechanism of action of cipargamin as an antibabesial drug candidate

**Contact Information:** [[e-mail]](mailto:[e-mail]); [[e-mail]](mailto:[e-mail])

## **Overview**

This dataset accompanies the publication titled \"Efficacy and mechanism of action of cipargamin as an antibabesial drug candidate.\" It includes data files corresponding to the figures and supplementary materials in the article. Each file is described below, along with variable definitions, experimental context, and processing methods. This README is designed to be a standalone guide, enabling users to interpret the data without referring to the original publication.

## **File Descriptions**

Several .csv files are combined figures. Each subfigure is separated by empty rows. Subfigures are explained as needed.

### **Figure 1.csv**

**1. Figure 1A & B: Growth data**

**Context:**

Dose-dependent growth inhibition of CIP on *B. bovis* and *B. gibson...,