Investigation of perturbation and effect of the bovine ocular microbiome

An experimental sub population consisting of 30 heifers was utilized to evaluate the effect of perturbation of one eye on the bacterial community composition of the other eye. This design included, swabbing of both the left and right eye in 10 animals on day 0 (cohort A), swabbing of the left eye in 10 animals on day 0 (cohort B), and swabbing of right eye in 10 animals on day 0 (cohort C), followed by swabbing of left and right eye in all animals for a second time 17 days later (n=30). Such design was set up to confirm findings from a longitudinal study.