Liver gene expression profiling of lipoprotein lipase heterozygous knockout mice

Lipoprotein lipase (Lpl) was predicted as a causal gene for abdominal using a novel statistical method named LCMS (Schadt et al., 2005, Nature Genetics). In order to validate this prediction, we profiled the liver tissues of lipoprotein lipase heterozygous knockout mice (Lpl+/-) and their littermate wild-type (wt) controls to examine the gene expression signature as well as pathways/networks resulting from the single gene perturbation. 8 Lpl+/- mice and 8 wt controls were profiled. Reference pool included RNA extracted from the liver of 9 wt control mice. Dye-swap was involved in the profiling.本研究借助一种名为LCMS的新型统计方法，将脂蛋白脂酶（Lipoprotein lipase, Lpl）预测为腹部相关致病基因（Schadt等，2005，《自然·遗传学》）。为验证该预测结论，我们对脂蛋白脂酶杂合敲除小鼠（Lpl+/-）及其同窝野生型（wild-type, wt）对照的肝脏组织开展基因表达谱分析，以考察单基因扰动引发的基因表达特征及相关通路与调控网络。本次分析共纳入8只Lpl+/-小鼠与8只wt对照小鼠。参考样本池包含9只wt对照小鼠肝脏提取的RNA。本次表达谱分析采用了染料交换实验设计。