Ct values and final intensities from triplicate nested TaqMan Real-Time PCR reactions for packed cell samples from wild honeycreepers with confirmed Avipoxvirus infections.

Successful amplifications (Ct425) are indicated in bold. All values are based on PCR Base Line Subtracted Curve Fit Data as calculated using iCycler version 3.1 software (BioRad). The threshold intensity was 23.4 rfu for the first reaction, 51.4 rfu for the second reaction, and 41.7 rfu for the third reaction.1Samples are identified by species, island and year of capture. Abbreviations are as follows: HAAM, Hawaìi `amakihi (Hemignathus virens); IIWI, ìiwi (Vestiaria coccinea); APAP, `apapane (Himatione sanguinea); HI, Hawaìi; MO, Molokài.2Laboratory Avipoxvirus isolates cultured from these individuals are included in Jarvi, et al., 2008 [8].3NR indicates the sample was not included in that reaction, a dash (−) indicates a failed first reaction and potential false negative, N/A indicates that no Ct value was assigned because the signal for the sample never reached the threshold intensity for that reaction.