PRDM16 Functions as A Compact Myocardium-Enriched Transcription Factor Required to Maintain Compact Myocardial Cardiomyocyte Identity in Left Ventricle (ChIP-seq)

Identification of genome-wide PRDM16 binding sites in E13.5 whole mouse heart, as well as in isolated left ventricle (LV) and right ventricle (RV). Overall design: Whole heart (two WT replicates), isolated LV (two WT replicates) and RV (two WT replicates) tissue samples were used in PRDM16 ChIP-seq experiments. Prdm16cKO samples and input samples were used to evaluate the specificity of PRDM16 antibody in ChIP-seq. Each whole heart sample combined three individual hearts of the same genotype, each isolated LV or RV samples combined 10-12 individual WT LVs or RVs.