Mouse single positive thymocyte bulk RNA-seq from Kmt2d knockout and control littermates.

To evaluate the hypothesis that dysregulated thymocyte development contributes to immune deficiencies in individuals with KS1. We generated bulk murine CD4+ or CD8+ single positive thymocyte RNA-seq data from cells which lack the histone methyltransferase, Kmt2d SET domain (exons 50/51), with a conditional Cre-recombinase driven by Lck-CreMar (T cell specific) or Vav1-iCre (hematopoietic). Expression profiling by high throughput sequencing. Overall design: 1) RNA-sequencing from 3 biological replicates of paired single positive (SP) thymocytes from Cre-recombinase (Lck-CreMar-driven: CD4+ and CD8+ SP thymocytes; or Vav1-iCre-driven: CD8+ SP thymocytes) Kmt2d knockout single positive thymocytes and corresponding Cre-negative control littermate. 2) Re-analysis of 7 Lck-Cre; Dot1l control and knockout CD8+ thymocyte RNA-Seq samples (GSE138910; GSM4121514-GSM4121516, GSM4121521-GSM4121524) and Kmt2d control and knockout peripheral naive CD4+ T cells RNA-seq (GSE69162; GSM1694174-GSM1694177) and ChIP-Seq (GSE69162; GSM1694154, GSM1694155) samples.