PAX5 controls lineage transitions between neuroendocrine and non-neuroendocrine states during carcinoma progression

Single cell lung suspensions of SCLC tissues were generated. scRNA-seq was performed on CD45 and CD31 negative live cells sorted by FACS. Purified cells were clustered to evaluate the cancer cell sub-clusters. Overall design: To study the intratumoral heterogeneity, we isolated individual cells (CD45/CD31-) from one freshly resected human primary SCLC, removed inflammatory infiltrate and endothelial cells, and performed single cell analysis using the 10x Genomics Chromium platform.