The dual role of Irf1 in maintaining epithelial identity while enabling EMT [mRNA-seq]

To investigate the context-dependent function of Irf1 in maintaining epithelial identity while enabling TGFbeta-induced EMT in NMuMG/E9 cells, we downregulated Irf1 by siRNA and analyzed differentially regulated genes and pathways upon EMT induction (2 days TGFbeta) or in the absence of EMT (0 day TGFbeta). Intersection with Irf1 ChIP-sequencing after 2 days of TGFbeta treatment or in untreated cells revealed genes that are directly regulated by Irf1 and that could contribute to the dual role of Irf1 in EMT. RNA sequencing of NMuMG/E9 cells transfected with Irf1-specific or control siRNA with (2 days) or without TGFbeta treatment to induce EMT.