Next Generation Sequencing and Analysis of the Transcriptomes of RAW264.7 cells pretreated with PEP and PBS.

We demonstrate that phosphoenolpyruvate (PEP) generally inhibits viral infection in vitro and in vivo. In order to further explore whether PEP controls viral replication through a general mechanism such as enhancing innate response, and if not, what's the critical downstream target(s) of PEP, we performed RNA sequencing (RNA-seq) analysis on the RAW264.7 cells that were pretreated with 250 μM PEP or PBS for 2 hours, and then infected with or without vesicular stomatitis virus (VSV) for 8 hours. Transcriptomes profiles of gene expression changes in RAW264.7 cells pretreated with 250 μM PEP and PBS for 2 hours, followed by VSV infection for 8 hours or without infection. Three biological replicates.