Screening of formulations of the plant-derived sterilizing agent ND-2 and its anti-fertility effect in mice

Organ coefficients were calculated using the following formula: organ coefficient = (organ mass/animal carcass mass) x 100% Sperm count was calculated by counting the number of spermatozoa in 25 squares around and in the center of a total of 5 squares under a light microscope using a hemacytometer. Sperm density was calculated using the following formula: Sperm density (total number of sperm/ml) = sperm count × dilution × 5 × 10000