FIGURE 4 RAW DATA MYD88 MUTANT TRACES

Fluorescence
time-traces obtained with disease-associated point mutants of the full-length
MyD88 protein, as well as the wild-type full-length MyD88 at a protein
concentration of 150 nM. Diffusion
time (µs)
measured by FCS ADDED ALREADY INTO FIGSHARE FILE FIGURE 2 RAW DATA showing the drastic shift in diffusion time when comparing the
mutants to the wild-type protein. (C)
Fluorescence intensity histogram showing that the L93P point mutation, which is
within the DD, in GFP-tagged MyD88 renders the polymerisation propensity
similar to the MyD88 TIR domain alone. (D) Fluorescence intensity
histogram demonstrating that the R196C and L252P point mutations (present
within the TIR domain) in the GFP-tagged MyD88 render the polymerisation
propensity more similar to the MyD88 DD alone. Fluorescence
time-traces and intensity histograms in (A), (C) & (D) are representative
of 8 independent experiments. Values in (B) are ±SD from these 8 measurements.