Unraveling the routes to distant metastases in colorectal cancer: Tumor deposits and lymph node metastases as the gateway

For this study, PCRs were performed with custom made primers for different polyG regions. Here, we include the exported quantified electropherograms from GeneMarker (V2.6.7, Sofgenetics) for the different polyG regions. Due to the repetitive nature of the PolyG tracts, the slippage of the polymerase generates a distribution of fragments (different base pairs) during the PCR, and this full peak pattern is used for the phylogenetic analyses. The data include an excel file for every case from the study with individual tabs for all primers used for the analysis of that specific case. For further information on methods see associated publication.