A 33-color panel for phenotypic analysis of murine organ specific immune cells

This 33-color panel delineates immune phenotypes of the major lymphocyte subsets in murine tissues
Conclusion:
The 33-color panel using Full Spectrum Flow Cytometry identified major subpopulations of lymphocytes in mouse organs with good resolution. This panel will be useful to identify and quantify the major lymphocytes populations for different mouse organs in autoimmune diseases or cancer.
Notes:
The 33-color panel using Full Spectrum Flow Cytometry identified major subpopulations of lymphocytes in mouse organs with good resolution. This panel will be useful to identify and quantify the major lymphocytes populations for different mouse organs in autoimmune diseases or cancer. Daily QC was performed using SpectroFlo® QC beads prior to acquiring samples to ensure that the cytometer was performing optimally, Antibody titrations for selection of optimal titers to reduce spillover spreading error, FMO stain controls for some weakly expressed antigens to exclude the influence from other fluorochromes.