Antibodies and nuclear dyes.
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Abbreviations: Cldn, claudin; JAM, junctional adhesion molecule; IF, immuno-fluorescence; Ocln, occludin; TG1, transglutaminase 1; ZO, zonula occludens.IF treatment for TG1: Citrate buffer, 2×10 min microwave, 0.001% trypsin, DAKO Block overnight. IF treatment for all others: TEC buffer, 2×10 min microwave, 0.001% trypsin, DAKO block.
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2015-12-02
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