Red Sea marine sponge Stylissa carteri metatranscriptomes. sponge metagenome

The primary goal of this study was to reveal the most transcribed gene functions of the prokaryotes associated with S. carteri. Stylissa carteri specimens (n=3) were collected at Fsar reef (22°23’N; 39°03’E) at the coast of Thuwal, Saudi Arabia. The collection occurred with water temperature at 30 °C, at 13-14 meters depth, on 2nd November 2010. Enriched prokaryotic mRNA was obtained from total RNA extracted from RNAlater-preserved sponge tissues. Enriched prokaryotic mRNA was amplified and pair-ended sequenced using Illumina HiSeq 2000 standard protocols.