Chronic exposure of mouse primary PAC to nicotine reduces the levels of TPKase proteins (A) and mRNA (B).

Western blot analysis was carried out using pancreatic acinar whole cell proteins (60 μg) isolated from chronic nicotine exposed mice and their controls. Real-time PCR was performed using mice gene-specific primers. Data are mean ± SE from separate sets of samples from multiple mice and were normalized relative to ARPO and calculated by the relative relationship method. *P < 0.01, **P < 0.05.