DDX5 in colonic tumors

The Asp-Glu-Ala-Asp (DEAD)-box RNA binding protein, DDX5, is ubiquitously expressed in many cell types, yet its role in RNA metabolism and its downstream function in vivo has not been fully elucidated. In the intestine, DDX5 is highly expressed in the intestinal epithelial cell (IECs) and contributes to tumorigenesis. Here, we used a conditional mouse lines where DDX5 is knocked out in IECs upon Tamoxifen administration to uncover mechanisms underlying DDX5 functions in colon tumors. For tumor collection, 8 week old littermates were treated with Tamoxifen on day 1, 2, 3 and tumor tissues were harvested betwee day 30-37 post first Tamoxifen (TAM) administration.