Immuhistochemical analyses of lipid droplets and metabolic factors in the BTBR mouse model of autism placenta

This dataset contains the underlying raw and processed data for the characterization of the mid-gestation (12.5 days post coitum) placenta in the BTBR T+ Itpr3tf/J mouse model. The study utilizes high-resolution 3D confocal microscopy to examine the spatial distribution of lipid droplets, the phospholipase PLA2G4E, and histone modifications (H3K9bu and H3K4me3) across distinct placental compartments. The results identify a strain-specific metabolic-epigenetic profile, particularly localized within trophoblast giant cells, which may serve as a foundational indicator for altered neurodevelopmental trajectories. The provided Dataset Summary Table offers a consolidated view of all quantitative measurements extracted from our image analysis pipeline, including lipid droplet volumetric data, field fluorescence intensities, and single-nuclei relative intensity values across all placental compartments. To ensure full technical transparency, we have included representative raw confocal stacks in .nd2 format, which contain the original multi-channel 3D signals for BODIPY 493/503, PLA2G4E, H3K9bu, and H3K4me3. These are complemented by representative Imaris (.ims) files that illustrate the specific 3D segmentation, surface generation, and thresholding masks used to derive our parameters.