Flow cytometry enumeration of virus-like and bacteria-like abundance in Ace, Deep, & Organic lakes (Antarctica)

Varying depth samples from Ace, Deep, & Organic Lake were obtained between 2013-2014. These samples were size fractionated by filtration for metagenomic analyses. Viral and bacterial abundances were determine on the filtrate fractions by flow cytometric analysis. Glutaraldehyde-fixed samples were stained with SYBR Green I fluorescence dye and virus-like particle (VLP) and bacteria-like cell (BLC) populations were discriminated based on green fluorescence and side scatter flow cytometry signals. Several distinct VLP and BLC populations where enumerated from Ace Lake and Organic Lake samples that revealed temporal and depth differences. Discrete VLP and BLC populations could not be discerned from Deep Lake samples. These files include the original flow cytometry particle counts (raw data), the gating templates used to count VLP and BLC populations (analysis_templates), and the resulting particle counts (analyzed).