Single cell RNA-seq developmental series in mouse hypothalamus in wild-type and Eed-cKO

Neuronal sub-type diversity in the hypothalamus is staggeringly immense. To investigate the role of epigenetic regulation in hypothalamic development in the generation of this cellular diversity; a floxed Eed allele was crossed to the early, pan-CNS Cre deleter Sox1-Cre, resulting in loss of H3K27me3 in the entire Mus musculus CNS by E11.5. E12.5, E13.5, E15.5, and E16.5 embryonic mouse hypothalamic cells were dissected and analysed by single cell RNA sequencing, on the Illimina/BioRad platform. Control and mutant Eed conditional knock-out at embryonic time points E12.5, E13.5, E15.5, and E16.5 were analysed by scRNA-seq in the mouse hypothalamus.下丘脑的神经元亚型多样性极为惊人。为探究表观遗传调控(epigenetic regulation)在下丘脑发育过程中对该细胞多样性形成的作用，研究人员将携带floxed Eed等位基因(floxed Eed allele)的小鼠与早期全中枢神经系统(pan-CNS) Cre重组酶工具鼠Sox1-Cre进行杂交，最终在胚胎发育第11.5天(E11.5)实现小家鼠(Mus musculus)全中枢神经系统内组蛋白H3赖氨酸27三甲基化(H3K27me3)的完全缺失。研究人员对胚胎发育第12.5天(E12.5)、第13.5天(E13.5)、第15.5天(E15.5)及第16.5天(E16.5)的小鼠下丘脑细胞进行解剖分离，并依托Illumina/BioRad测序平台开展单细胞RNA测序(single cell RNA sequencing, scRNA-seq)分析。本数据集同时针对上述四个胚胎时间点的对照组与Eed条件性敲除(conditional knock-out)突变体小鼠的下丘脑组织，完成了单细胞RNA测序分析。