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Gut microbiome of diabetes

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NIAID Data Ecosystem2026-03-12 收录
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Total DNA of microorganisms was extracted from all feces samples using a QIAamp stool DNA minikit (Qiagen, Germany). The extracted genomic DNA was used to construct an amplicon library by amplifying the V3~V4 region of the 16S rRNA gene. An Illumina Miseq high-throughput sequencing platform (Illumina) was used to sequence the PCR products in the 16S RNA V3~V4 region.

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2021-02-05
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