Transcription profiling of mouse pooled embryonic humeri E14.5 from wild type and RUNX2-/- mutants detects novel skeletogenesis target genes

We used microarrays to identify genes differentially expressed between mouse RUNX2 -/- and wt embryonic humeri at stage E14.5 Experiment Overall Design: To minimize the effects of random biological variation, humeri from different preparations were pooled (approximately 75 wildtype and 60 Runx2-/- for each of the two biological replicates).本研究利用基因芯片（microarrays）筛选小鼠E14.5阶段胚胎肱骨中RUNX2 -/-与野生型（wt）样本间的差异表达基因。实验总体设计：为最小化随机生物学变异的影响，将不同制备批次的肱骨样本进行混合，两个生物学重复样本各自包含约75份野生型样本与60份RUNX2 -/-样本。