Supplementary data: Role of miRNAs in the function of restoration of severe congenital neutropenia associated genes

Supplementary Figure S1. Visual representation of the regulatory network generated in BioNet UCR, by adding FORWARD and BACKWARD genes (HAX1, JAGN1, WAS, G6PC3) and deleting source and autoregulated nodes. Supplementary Figure S2. Differential expression analysis done in Cytoscape program to verify which nodes are regulated negatively or positively by their LogFC values. Supplementary Figure S3. Result of the parameter estimation task from CopasiUI applied to the model. Particle swarm algorithm was applied, showing a fitted model with no species going under the 0.05 threshold. Supplementary Table S4. HAX1 simulated mutation data related to its associated CNV MIR 6864, whereas 0.5 CNV is an approximate point where the function of the gene (concentration) can be restored. Supplementary Table S5. JAGN1 simulated mutation data related to its associated CNV MIR 6515, whereas 1.05 CNV is an approximate point where the function of the gene (concentration) can be restored. Supplementary Figure S6. Proposal of experimental design of an ex vivo cellular model, in which the results of the in silico model can be measured in a quantitative way.