Gene expression levels of evolved thermotolerant yeast and its parent under fermentation condition

Purpose: To explore the differentially expressed genes and pathways in the thermotolerant mutant compare to wildtype. To figure out the contribution of these genes and pathways to high protein expression ability in the mutant. Method: the samples were collected from the 60h fermentation culture. The fermentation medium was BMGY/BMMY with 1%(v/v) methanol as inducer. the cultivation condition was set under 28℃ and 37℃. Results: after data analysis, we found genes related to oxidative stress response were important. Examination of 3 groups of sample under fermentation condition.