Saccharomyces cerevisiae Genome and Transciprtome Sequencing

We leveraged PacBio's long read sequencing technology to generate de novo assemblies of two wild Saccharomyces cerevisiae strains (Y12 and DBVPG6044) as well as deep RNA-Seq data (>1000X) to facilitate unbiased genome annotation. Our high-quality de novo genome assemblies capture the entire spectrum of genetic variation and resolve structurally complex genomic regions. Additionally, our unbiased genome annotations provide an accurate parts list of the genes present in each strain. Using these assemblies, we also evaluated how de novo genome assemblies mitigate read mapping bias in estimates of allele specific expression measured in Y12 x DBVPG6044 diploids.