DC-SIGN expression on myeloid population in Luminal Breast Cancer

DC-SIGN expression by the different populations of DC and macrophages found in breast tumor samples using flow cytometry. As previously described (doi:10.1038/s41590-018-0145-8.), we identified four major DC populations: CD11c − CD123+ plasmacytoid pre-DCs, CD11c+BDCA1+ CD14− DCs, CD11c+ BDCA1− CD14− DCs, and CD11c+ BDCA1+ CD14+ inflammatory DCs. Staining was performed using the following antibodies: anti-CD45 APC-Cy7 (BD), anti-CD14 Qdot 605 (Thermo), anti-CD3 Alexa700 (Biolegend), anti-CD 19 Alexa700 (Biolegend), anti-CD56 Alexa700 (Biolegend), 400 anti-HLA-DR BV711 (Biolegend), anti-CD11c PC5 (Beckman Coulter), anti-BDCA-1 PE (BD), anti-CD123 PCy7 (BD) and anti-C-SIGN FITC (BD). Cells were analyzed by a LSRFORTESSA X-20 instrument (BD Biosciences). The most prominent intratumoral myeloid cell population, however, was shown to be represented by macrophages, defined as CD11c+ BDCA1−CD14+ cells. We analyzed DC-SIGN expression on the surface of these populations by flow cytometry. DC-SIGN was only expressed on the membrane of macrophages from tumoral as well as juxtatumoral tissues, but not on the DC populations analyzed. These results are represented in Figure 4 from the publication "Aberrant fucosylation enables breast cancer clusterin to interact with dendritic cell-specific ICAM-grabbing non-integrin (DC-SIGN)".