Binding and regulation of transcription by yeast Ste12 variants to drive mating and invasion phenotypes

Here, we examine how six single amino acid variants in the DNA-binding domain of Ste12 – a yeast transcription factor regulating mating and invasion – alter Ste12 genome binding, motif recognition and gene expression to yield markedly different phenotypes. Using a combination of the calling card method, RNA sequencing , we find that variants with dissimilar binding and expression profiles can converge onto similar cellular behaviors.本研究探讨了酿酒酵母转录因子Ste12的DNA结合域（DNA-binding domain）内6种单氨基酸变异——该转录因子调控酵母的交配与侵袭过程——如何改变Ste12的基因组结合能力、基序（motif）识别特性与基因表达模式，进而催生显著迥异的表型。本研究结合召唤标签法（calling card method）与RNA测序（RNA sequencing）技术，发现结合谱与表达谱存在差异的变异体，可趋同至相似的细胞行为。