Metagenomic Study of Respiratory Tract Microbial Community Change During Long-Term Acute Care

We applied metagenomic sequencing to respiratory and gastrointestinal tract specimens, collected from a cohort of long-term acute care hospital (LTACH) residents, in order to define microbiome and resistome change under antibiotic pressure, generate microbiome disruption indices (MDIs) to predict risk for multidrug-resistant infection, and contribute to a deeper understanding of how antibiotics and other medications increase risk of specific AMR gene selection and persistence.